| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (BA14789).
Lane 1: recombinant rat IL-1 beta/IL1B protein 10 ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL-1 beta/IL1B antigen affinity purified polyclonal antibody (BA14789) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL-1 beta/IL1B at approximately 17 kDa.

Western blot analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (BA14789).
Lane 1: recombinant rat IL-1 beta/IL1B protein 10 ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL-1 beta/IL1B antigen affinity purified polyclonal antibody (BA14789) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL-1 beta/IL1B at approximately 17 kDa.


