Anti-IL-1 beta/IL1B Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC ELISA

PB0055

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (PB0055). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL-1 beta/IL1B antigen affinity purified polyclonal antibody (PB0055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL-1 beta/IL1B at approximately 35 kDa. The expected band size for IL-1 beta/IL1B is at 31 kDa.
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Figure 2. Western blot analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (PB0055).
Lane 1: recombinant rat IL1B protein 10 ng,
Lane 2: recombinant rat IL1B protein 5 ng
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL-1 beta/IL1B antigen affinity purified polyclonal antibody (PB0055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL-1 beta/IL1B at approximately 17 kDa. The expected band size for IL-1 beta/IL1B is at 31 kDa.
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Figure 3. IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (PB0055) .
IL-1 beta/IL1B was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-IL-1 beta/IL1B Antibody (PB0055) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (PB0055).
IL-1 beta/IL1B was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-IL-1 beta/IL1B Antibody (PB0055) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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产品简介>

产品名称

Anti-IL-1 beta/IL1B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

IL1 beta; Catabolin; IL 1; IL 1 beta; IL1 BETA; IL-1 beta; IL1- beta; IL1B; IL1beta; IL1F2; IL-1β; Interleukin 1 beta; interleukin 1; beta; Pro IL-1β; IL 1B; IL-1B

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA

基因名称

IL1B

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of rat IL-1 beta different from the related human sequence by five amino acids, and from the related mouse sequence by one amino acid.

计算分子量

31 kDa

实际分子量

31-35 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Uniprot ID

Q63264

文献引用格式

IL-1 beta/IL1B Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0055)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 1, beta

蛋白名全称

Interleukin-1 beta

实验方案>

实验步骤

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