Western blot (WB): | 1:500-2000 |
ImmunoPrecipitation (IP): | 1:50 |
Flow Cytometry (FCM): | 1:50 |
Western blot analysis of IL1B using anti-IL1B antibody (M00101-3).
Lane 1: recombinant rat IL1B protein.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL1B antigen affinity purified monoclonal antibody (M00101-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL1B at approximately 17 kDa.
Western blot analysis of IL1B using anti-IL1B antibody (M00101-3).
Lane 1: recombinant rat IL1B protein.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL1B antigen affinity purified monoclonal antibody (M00101-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL1B at approximately 17 kDa.