Anti-GAPDH Antibody (Clone#5A12)

Mouse Monoclonal Antibody

说明书

筛选器： All WB ICC/IF IP

BM1623

100μl ￥880 200μl ￥1680 1ml ￥6800
货期：现货

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Figure 1. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-GAPDH Antibody (Clone#5A12)

规格/价格

100μl/880 200μl/1680 1ml/6800

指标别称

G3PD; GAPD; GAPDH; OK/SW cl.12

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey, zebrafish, chicken, rabbit, pig

应用范围

WB, ICC/IF, IP

基因名称

GAPDH

抗体来源

Mouse

抗体亚型

IgG1

免疫原

Polypeptide

计算分子量

36 kDa

实际分子量

36 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA

纯化方式

protein G purified.

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P04406

RRID

AB_2885058

文献引用格式

GAPDH Antibody (Clone#5A12) (Boster Biological Technology, Wuhan, China. Catalog#BM1623)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Glyceraldehyde-3-phosphate dehydrogenase

实验方案>

实验步骤

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