Anti-Arginase-1/ARG1 Antibody (Clone#ABO-1)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP

BM3973

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-Arginase-1/ARG1 antibody (BM3973). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Arginase-1/ARG1 antigen affinity purified monoclonal antibody (BM3973) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Arginase-1/ARG1 at approximately 35-40 kDa. The expected band size for Arginase-1/ARG1 is at 35 kDa.
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Immunohistochemical analysis of paraffin-embedded human liver, using Liver Arginase Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Arginase-1/ARG1 Antibody (Clone#ABO-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARG1; Arginase 1; Arginase-1; liver Arginase; Liver type arginase

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP

基因名称

ARG1

克隆号

ABO-1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Liver Arginase

计算分子量

35 kDa

实际分子量

35-40 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ARG1(arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1℃DNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P05089

文献引用格式

Arginase-1/ARG1 Antibody (Clone#ABO-1) (Boster Biological Technology, Wuhan, China. Catalog#BM3973)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Arginase-1

实验方案>

实验步骤

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