Anti-Arginase-1/ARG1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

PB0525

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Arginase-1/ARG1 using anti-Arginase-1/ARG1 antibody (PB0525). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Arginase-1/ARG1 antigen affinity purified polyclonal antibody (PB0525) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Arginase-1/ARG1 at approximately 38 kDa. The expected band size for Arginase-1/ARG1 is at 35 kDa.
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产品简介>

产品名称

Anti-Arginase-1/ARG1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARG1; Arginase 1; Arginase-1; liver Arginase; Liver type arginase

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

ARG1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human liver Arginase different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

计算分子量

35 kDa

实际分子量

38 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P05089

文献引用格式

Arginase-1/ARG1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0525)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arginase, liver

蛋白名全称

Arginase-1

实验方案>

实验步骤

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