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Anti-Arginase-1/ARG1 Antibody (Clone#OTI4E6)

Mouse monoclonal antibody

说明书

MA01106

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01106)

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  • Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARG1 monoclonal antibody.

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  • Figure 3. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARG1 antibody, and then analyzed by flow cytometry.

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  • Figure 4. Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ARG1 monoclonal antibody (1:200).

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  • Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARG1(Cat# MA01106).

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产品名称
Anti-Arginase-1/ARG1 Antibody (Clone#OTI4E6)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ARG1; Arginase 1; Arginase-1; liver Arginase; Liver type arginase
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, FCM
基因名称
ARG1
克隆号
OTI4E6
抗体来源
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human ARG1 (NP_000036) produced in HEK293T cell.
计算分子量
35 kDa
实际分子量
34.6 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
Uniprot ID
P05089  
文献引用格式
Arginase-1/ARG1 Antibody (Clone#OTI4E6) (Boster Biological Technology, Wuhan, China. Catalog#MA01106)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
arginase, liver
蛋白名全称
Arginase-1
推荐稀释比
Western blot (WB):1:1000~2000
Immunohistochemistry (IHC):1:50
Flow cytometry (FCM):1:100

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