Anti-Arginase-1/ARG1 Antibody (Clone#2B12)

筛选器： All WB FCM

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  Figure 1. Western blot analysis of Arginase-1/ARG1 using anti-Arginase-1/ARG1 antibody (M01106-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: mouse liver tissue lysates,
  Lane 3: monkey liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Arginase-1/ARG1 antigen affinity purified monoclonal antibody (M01106-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Arginase-1/ARG1 at approximately 35 kDa. The expected band size for Arginase-1/ARG1 is at 35 kDa.

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  Figure 2. Flow Cytometry analysis of Jurkat cells using anti-Arginase-1/ARG1 antibody (M01106-4).
  Overlay histogram showing Jurkat cells stained with M01106-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Arginase-1/ARG1 Antibody (M01106-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 3. Flow Cytometry analysis of SiHa cells using anti-Arginase-1/ARG1 antibody (M01106-4).
  Overlay histogram showing SiHa cells stained with M01106-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Arginase-1/ARG1 Antibody (M01106-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of Arginase-1/ARG1 using anti-Arginase-1/ARG1 antibody (M01106-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: mouse liver tissue lysates,
  Lane 3: monkey liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Arginase-1/ARG1 antigen affinity purified monoclonal antibody (M01106-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Arginase-1/ARG1 at approximately 35 kDa. The expected band size for Arginase-1/ARG1 is at 35 kDa.

• 

  Figure 2. Flow Cytometry analysis of Jurkat cells using anti-Arginase-1/ARG1 antibody (M01106-4).
  Overlay histogram showing Jurkat cells stained with M01106-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Arginase-1/ARG1 Antibody (M01106-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Figure 3. Flow Cytometry analysis of SiHa cells using anti-Arginase-1/ARG1 antibody (M01106-4).
  Overlay histogram showing SiHa cells stained with M01106-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Arginase-1/ARG1 Antibody (M01106-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.