Anti-P53/TP53 (Phospho-S392) Antibody (Clone#BDO-20)

Rabbit Monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP

BM4095

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Figure 1. Western blot analysis of anti-P53/TP53 antibody (BM4095). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P53/TP53 antigen affinity purified monoclonal antibody (BM4095) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P53/TP53 at approximately 53 kDa. The expected band size for P53/TP53 is at 44 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody.
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Immunofluorescent analysis using the Antibody.
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产品名称

Anti-P53/TP53 (Phospho-S392) Antibody (Clone#BDO-20)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Antigen NY CO 13; Cellular tumor antigen p53; LFS1; P53; Phosphoprotein p53; TP53; TRP53; tumor protein p53; Tumor suppressor p53

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

TP53

克隆号

BDO-20

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-p53 (S392)

计算分子量

44 kDa

实际分子量

53 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Uniprot ID

P04637

文献引用格式

P53/TP53 (Phospho-S392) Antibody (Clone#BDO-20) (Boster Biological Technology, Wuhan, China. Catalog#BM4095)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cellular tumor antigen p53

实验方案>

实验步骤

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