产品名称
Anti-P53/TP53 Antibody (Clone#OTI3D5)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Antigen NY CO 13; Cellular tumor antigen p53; LFS1; P53; Phosphoprotein p53; TP53; TRP53; tumor protein p53; Tumor suppressor p53
应用范围
WB, IHC, ICC/IF, FCM
免疫原
Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.
文献引用格式
P53/TP53 Antibody (Clone#OTI3D5) (Boster Biological Technology, Wuhan, China. Catalog#M00001-6)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
蛋白名全称
Cellular tumor antigen p53
推荐稀释比
Western blot (WB): | 1:500~2000 |
Immunohistochemistry (IHC): | 1:150 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |