Anti-P53/TP53 Antibody (Clone#AGC-20)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4026

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of P53/TP53 using anti-P53/TP53 antibody (BM4026). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-9(WT) whole cell lysates,
Lane 2: human PC-9(KO) whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P53/TP53 antigen affinity purified monoclonal antibody (BM4026) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P53/TP53 at approximately 53 kDa. The expected band size for P53/TP53 is at 44 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using p53 Antibody.
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Immunofluorescent analysis of Hela cells, using p53 Antibody .
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产品简介>

产品名称

Anti-P53/TP53 Antibody (Clone#AGC-20)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Antigen NY CO 13; Cellular tumor antigen p53; LFS1; P53; Phosphoprotein p53; TP53; TRP53; tumor protein p53; Tumor suppressor p53

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP

基因名称

TP53

克隆号

AGC-20

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human p53

计算分子量

44 kDa

实际分子量

53 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Uniprot ID

P04637

文献引用格式

P53/TP53 Antibody (Clone#AGC-20) (Boster Biological Technology, Wuhan, China. Catalog#BM4026)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cellular tumor antigen p53

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实验步骤

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