Anti-P53/TP53 Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of P53/TP53 using anti-P53/TP53 antibody (PB9008). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human 293T whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P53/TP53 antigen affinity purified polyclonal antibody (PB9008) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P53/TP53 at approximately 53 kDa. The expected band size for P53/TP53 is at 44 kDa.

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  Figure 2. IHC analysis of P53/TP53 using anti-P53/TP53 antibody (PB9008).
  P53/TP53 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-P53/TP53 Antibody (PB9008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IF analysis of P53/TP53 using anti-P53/TP53 antibody (PB9008).
  P53/TP53 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-P53/TP53 Antibody (PB9008) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.

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  Figure 4. Flow Cytometry analysis of A431 cells using anti-P53/TP53 antibody (PB9008).
  Overlay histogram showing A431 cells stained with PB9008 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P53/TP53 Antibody (PB9008) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Figure 1. Western blot analysis of P53/TP53 using anti-P53/TP53 antibody (PB9008). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human 293T whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P53/TP53 antigen affinity purified polyclonal antibody (PB9008) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P53/TP53 at approximately 53 kDa. The expected band size for P53/TP53 is at 44 kDa.

• 

  Figure 2. IHC analysis of P53/TP53 using anti-P53/TP53 antibody (PB9008).
  P53/TP53 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-P53/TP53 Antibody (PB9008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

• 

  Figure 3. IF analysis of P53/TP53 using anti-P53/TP53 antibody (PB9008).
  P53/TP53 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-P53/TP53 Antibody (PB9008) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.

• 

  Figure 4. Flow Cytometry analysis of A431 cells using anti-P53/TP53 antibody (PB9008).
  Overlay histogram showing A431 cells stained with PB9008 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P53/TP53 Antibody (PB9008) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.