Anti-AKT1 Antibody (Clone#EBG-1)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP FCM

BM4390

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- AKT1 antibody (BM4390). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEPG2 whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SIHA whole cell lysates,
Lane 7: 293T whole cell lysates.
Use rabbit Anti-AKT1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AKT1 at approximately 60KD. The expected band size for AKT1 is at 56KD.
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Figure 2. Western blot analysis of anti- AKT1 antibody (BM4390). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: C6 whole cell lysates,
Lane 4: RH35 whole cell lysates,
Lane 5:mouse liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: Neuro-2a whole cell lysates.
Lane 8: HEPA1-6 whole cell lysates.
Use rabbit Anti-AKT1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AKT1 at approximately 60KD. The expected band size for AKT1 is at 56KD.
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产品简介>

产品名称

Anti-AKT1 Antibody (Clone#EBG-1)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

AKT1

克隆号

EBG-1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

计算分子量

56 kDa

实际分子量

56-60 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Uniprot ID

P31749

文献引用格式

AKT1 Antibody (Clone#EBG-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4390)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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