Anti-AKT1 (Phospho-T450) Antibody (Clone#HEA-1)

Rabbit Monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP

BM4721

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-AKT1 antibody (BM4721). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (BM4721) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.
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Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
AKT1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
AKT1 was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
AKT1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 5. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
AKT1 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 6. IF analysis of AKT1 using anti-AKT1 antibody (BM4721) and anti-Beta Tubulin antibody (M01857-3).
AKT1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-AKT1 (Phospho-T450) Antibody (Clone#HEA-1)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

AKT1

克隆号

HEA-1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human AKT1

计算分子量

56 kDa

实际分子量

56-60 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Uniprot ID

P31749

文献引用格式

AKT1 (Phospho-T450) Antibody (Clone#HEA-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4721)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

RAC-alpha serine/threonine-protein kinase

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