Anti-NRF2/NFE2L2(Phospho-S40) Antibody (Clone#IFH-14)

Rabbit Monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4842

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-NRF2/NFE2L2 antibody (BM4842). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NRF2/NFE2L2 antigen affinity purified monoclonal antibody (BM4842) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NRF2/NFE2L2 at approximately 100 kDa. The expected band size for NRF2/NFE2L2 is at 68 kDa.
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IHC analysis of NRF2/NFE2L2 using anti-NRF2/NFE2L2 antibody (BM4842) .
NRF2/NFE2L2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-NRF2/NFE2L2 Antibody (BM4842) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of NRF2/NFE2L2 using anti-NRF2/NFE2L2 antibody (BM4842) .
NRF2/NFE2L2 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-NRF2/NFE2L2 Antibody (BM4842) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(10)
IHC analysis of NRF2/NFE2L2 using anti-NRF2/NFE2L2 antibody (BM4842) .
NRF2/NFE2L2 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-NRF2/NFE2L2 Antibody (BM4842) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HepG2 cells, using Phospho-Nrf2 (S40) Antibody.
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Western blot analysis of anti-NRF2/NFE2L2 antibody (BM4842). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NRF2/NFE2L2 antigen affinity purified monoclonal antibody (BM4842) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NRF2/NFE2L2 at approximately 100 kDa. The expected band size for NRF2/NFE2L2 is at 68 kDa.
all(10)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NRF2/NFE2L2(Phospho-S40) Antibody (Clone#IFH-14)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; NRF2; NRF2; NFE2L2

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

NFE2L2

克隆号

IFH-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-Nrf2 (S40)

计算分子量

68 kDa

实际分子量

100-110 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NFE2L2(nuclear factor (erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-directed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas(G12D) and BRaf(V619E), and in human pancreatic cancer.

Uniprot ID

Q16236

文献引用格式

NRF2/NFE2L2(Phospho-S40) Antibody (Clone#IFH-14) (Boster Biological Technology, Wuhan, China. Catalog#BM4842)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Nuclear factor erythroid 2-related factor 2

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实验步骤

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