Human GM-CSF/CSF2 ELISA Kit

96T/2480

Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim

E.coli, A18-E144

There is no detectable cross-reactivity with other relevant proteins.

15.6 pg/ml - 1000 pg/ml

CSF2

colony stimulating factor 2 (granulocyte-macrophage)

P04141  

Human GM-CSF/CSF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0364)

Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

15-20分钟

Catalog number	Description	Quantity
EK0364-CAP	96-well plate precoated with anti- human GM-CSF antibody	1
EK0364-ST	Lyophilized recombinant human GM-CSF standard	10ng/tube×2
EK0364-DA	Biotinylated anti- human GM-CSF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	28	213	388	27	219	407
Standard deviation	1.34	15.76	19.78	1.8	20.14	26.04
CV(%)	4.8	7.4	5.1	6.7	9.2	6.4

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.005	0.067	0.1300	0.245	0.513	0.927	1.655	2.274

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h