Rat GM-CSF/CSF2 ELISA Kit

96T/2480

Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim

Expression system for standard: E.coli; Immunogen sequence: A1-K127

There is no detectable cross-reactivity with other relevant proteins.

15.6pg/ml-1000pg/ml

CSF2

colony stimulating factor 2 (granulocyte-macrophage)

P48750  

Rat GM-CSF/CSF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0366)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Catalog number	Description	Quantity
EK0366-CAP	96-well plate precoated with anti- rat GM-CSF antibody	1
EK0366-ST	Lyophilized recombinant rat GM-CSF standard	10ng/tube×2
EK0366-DA	Biotinylated anti- rat GM-CSF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	30	216	429	28	196	414
Standard deviation	1.35	11.44	33.89	1.59	12.34	39.33
CV(%)	4.5%	5.3%	7.9%	5.7%	6.3%	9.5%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.030	0.085	0.138	0.229	0.423	0.806	1.391	2.271

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)