Human GM-CSF/CSF2 Quick ELISA Kit

96T/3580

Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim

Expression system for standard: E.coli; Immunogen sequence: A18-E144

There is no detectable cross-reactivity with other relevant proteins.

15.6pg/ml-1000pg/ml

CSF2

colony stimulating factor 2 (granulocyte-macrophage)

P04141  

Human GM-CSF/CSF2 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0364)

Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

15分钟

Catalog number	Description	Quantity
FEK0364-CAP	Anti-human GM-CSF Pre-coated 96-well Strip Microplate	1
FEK0364-ST	human GM-CSF standard	10ng/tube×2
FEK0364-H	HRP Linked Anti-human GM-CSF Antibody	6ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	28	213	388	27	219	407
Standard deviation	1.34	15.76	19.78	1.8	20.14	26.04
CV(%)	4.8%	7.4%	5.1%	6.7%	9.2%	6.4%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.022	0.053	0.109	0.229	0.515	0.932	1.536	2.216

Store at 4℃ for 6 months.

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)