产品名称
Mouse GM-CSF/CSF2 ELISA Kit
指标别名
Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim
免疫原
Expression system for standard: E.coli; Immunogen sequence: A18-K141
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Granulocyte-macrophage colony-stimulating factor
文献引用格式
Mouse GM-CSF/CSF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0365)
背景资料
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0365-CAP | 96-well plate precoated with anti- mouse GM-CSF antibody | 1 |
EK0365-ST | Lyophilized recombinant mouse GM-CSF standard | 10ng/tube×2 |
EK0365-DA | Biotinylated anti- mouse GM-CSF antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 32 | 234 | 480 | 35 | 213 | 448 |
Standard deviation | 1.34 | 16.38 | 19.68 | 1.54 | 14.91 | 21.05 |
CV(%) | 4.2% | 7% | 4.1% | 4.4% | 7% | 4.7% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.007 | 0.041 | 0.098 | 0.172 | 0.465 | 0.760 | 1.502 | 2.104 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)