Human IL4 ELISA Kit

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EK0404

96T ￥1880
货期：现货

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Human IL-4 ELISA Kit standard curve
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产品简介>

产品名称

Human IL4 ELISA Kit

规格/价格

96T/1880

指标别名

B cell stimulatory factor 1; Binetrakin; BSF 1; BSF1; IL 4; IL4; IL-4; interleukin 4; Pitrakinra

免疫原

E.coli, H25-S153

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

15.6 pg/ml - 1000 pg/ml

基因名称

IL4

基因名全称

interleukin 4

Uniprot ID

P05112

文献引用格式

Human IL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0404)

背景资料

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0404-CAP	96-well plate precoated with anti- human IL-4 antibody	1
EK0404-ST	Lyophilized recombinant human IL-4 standard	10ng/tubex2
EK0404-DA	Biotinylated anti- human IL-4 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	35	216	508	33	195	532
Standard deviation	2.03	12.52	21.33	2.04	11.89	29.26
CV(%)	5.8	5.8	4.2	6.2	6.1	5.5

典型数据示例

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.018	0.039	0.073	0.151	0.377	0.908	1.872	2.627

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human IL4 ELISA Kit

EK0404

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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Human IL4 ELISA Kit

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