产品名称
Human IL4 Quick ELISA Kit
指标别名
B cell stimulatory factor 1; Binetrakin; BSF 1; BSF1; IL 4; IL4; IL-4; interleukin 4; Pitrakinra
免疫原
Expression system for standard: E.coli; Immunogen sequence: H25-S153
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human IL4 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0404)
背景资料
Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0404-CAP | Anti-human IL-4 Pre-coated 96-well Strip Microplate | 1 |
| FEK0404-ST | human IL-4 standard | 10ng/tube×2 |
| FEK0404-H | HRP Linked Anti-human IL-4 Antibody | 6ml |
| AR1106-1 | Sample diluent buffer | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | Stop solution | 10ml |
| PLA-SEA | Adhesive cover | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 216 | 508 | 33 | 195 | 532 |
| Standard deviation | 2.03 | 12.52 | 21.33 | 2.04 | 11.89 | 29.26 |
| CV(%) | 5.8% | 5.8% | 4.2% | 6.2% | 6.1% | 5.5% |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.040 | 0.148 | 0.238 | 0.516 | 0.755 | 1.314 | 2.094 | 2.570 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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