指标别名
B cell stimulatory factor 1; Binetrakin; BSF 1; BSF1; IL 4; IL4; IL-4; interleukin 4; Pitrakinra
免疫原
Expression system for standard: E.coli; Immunogen sequence: C25-S147
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0406)
背景资料
Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.
试剂盒内容
Catalog number | Description | Quantity |
EK0406-CAP | 96-well plate precoated with anti- rat IL-4 antibody | 1 |
EK0406-ST | Lyophilized recombinant rat IL-4 standard | 10ng/tubex2 |
EK0406-DA | Biotinylated anti- rat IL-4 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 164 | 456 | 32 | 175 | 481 |
Standard deviation | 2.45 | 10.66 | 21.43 | 2.4 | 11.37 | 28.37 |
CV(%) | 7% | 6.5% | 4.7% | 7.5% | 6.5% | 5.9% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.057 | 0.106 | 0.159 | 0.263 | 0.517 | 1.043 | 1.781 | 2.383 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum