指标别名
B cell stimulatory factor 1; Binetrakin; BSF 1; BSF1; IL 4; IL4; IL-4; interleukin 4; Pitrakinra
免疫原
Expression system for standard: E.coli; Immunogen sequence: H21-S140
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0405)
背景资料
Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0405-CAP | 96-well plate precoated with anti- mouse IL-4 antibody | 1 |
EK0405-ST | Lyophilized recombinant mouse IL-4 standard | 10ng/tubex2 |
EK0405-DA | Biotinylated anti- mouse IL-4 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 15 | 96 | 280 | 16 | 93 | 275 |
Standard deviation | 1.11 | 6.62 | 20.44 | 1.48 | 6.69 | 20.9 |
CV(%) | 7.4% | 6.9% | 7.3% | 9.3% | 7.2% | 7.6% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.003 | 0.039 | 0.072 | 0.138 | 0.310 | 0.786 | 1.681 | 2.362 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum