产品名称
Human RANKL/TNFSF11 ELISA Kit
指标别名
CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
基因名全称
tumor necrosis factor (ligand) superfamily, member 11
文献引用格式
Human RANKL/TNFSF11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0842)
背景资料
Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0842-CAP | 96-well plate precoated with anti- human TNFSF11 antibody | 1 |
EK0842-ST | Lyophilized recombinant human TNFSF11 standard | 10ng/tube×2 |
EK0842-DA | Biotinylated anti- human TNFSF11 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 115 | 865 | 2242 | 114 | 797 | 2279 |
Standard deviation | 5.52 | 53.63 | 105.37 | 5.92 | 58.97 | 116.22 |
CV(%) | 4.8 | 6.2 | 4.7 | 5.2 | 7.4 | 5.1 |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.017 | 0.115 | 0.227 | 0.416 | 0.573 | 1.015 | 1.591 | 2.039 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)