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Human RANKL/TNFSF11 ELISA Kit

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EK0842

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human RANKL/TNFSF11 ELISA Kit
规格/价格
96T/2480
指标别名
CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE
免疫原
NSO, G64-D245
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
基因名称
TNFSF11
基因名全称
tumor necrosis factor (ligand) superfamily, member 11
Uniprot ID
O14788  
文献引用格式
Human RANKL/TNFSF11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0842)
背景资料
Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0842-CAP96-well plate precoated with anti- human TNFSF11 antibody1
EK0842-STLyophilized recombinant human TNFSF11 standard10ng/tube×2
EK0842-DABiotinylated anti- human TNFSF11 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)11586522421147972279
Standard deviation5.5253.63105.375.9258.97116.22
CV(%)4.86.24.75.27.45.1
典型数据示例
Concentration(pg/ml)078156313625125025005000
O.D.0.0170.1150.2270.4160.5731.0151.5912.039
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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    Human RANKL/TNFSF11 ELISA Kit

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