产品名称
Rat RANKL/TNFSF11 ELISA Kit
指标别名
CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE
免疫原
Expression system for standard: E.coli; Immunogen sequence: F141-D318
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
tumor necrosis factor (ligand) superfamily, member 11
文献引用格式
Rat RANKL/TNFSF11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1559)
背景资料
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. The rat gene is mapped to 15q11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
试剂盒内容
Catalog number | Description | Quantity |
EK1559-CAP | 96-well plate precoated with anti- rat TNFSF11 antibody | 1 |
EK1559-ST | Lyophilized recombinant rat TNFSF11 standard | 10ng/tube×2 |
EK1559-DA | Biotinylated anti- rat TNFSF11 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 37 | 209 | 464 | 38 | 208 | 445 |
Standard deviation | 2.25 | 8.56 | 28.76 | 2.96 | 12.06 | 35.15 |
CV(%) | 6.1% | 4.1% | 6.2% | 7.8% | 5.8% | 7.9% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.087 | 0.145 | 0.202 | 0.315 | 0.522 | 0.909 | 1.581 | 2.347 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)