产品名称
Human RANKL/TNFSF11 Quick ELISA Kit
指标别名
CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE
免疫原
Expression system for standard: NS0; Immunogen sequence: G64-D245
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
tumor necrosis factor (ligand) superfamily, member 11
文献引用格式
Human RANKL/TNFSF11 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0842)
背景资料
Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
试剂盒内容
Catalog number | Description | Quantity |
FEK0842-CAP | 96-well plate precoated with anti-Human TNFSF11 antibody | 1 |
FEK0842-ST | lyophilized recombinant Human TNFSF11 standard | 10ng/tube×2 |
FEK0842-A | Human TNFSF11 Antibody A | 3ml |
FEK0842-B | Human TNFSF11 Antibody B | 3ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 117 | 867 | 2244 | 116 | 799 | 2281 |
Standard deviation | 5.85 | 55.48 | 109.95 | 6.26 | 60.72 | 120.89 |
CV(%) | 5.0% | 6.4% | 4.9% | 5.4% | 7.6% | 5.3% |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
O.D. | 0.034 | 0.139 | 0.151 | 0.262 | 0.453 | 0.783 | 1.331 | 2.026 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)