产品名称
Mouse RANKL/TNFSF11 ELISA Kit
指标别名
CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE
免疫原
Expression system for standard: NS0; Immunogen sequence: R72-D316
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor ligand superfamily member 11
文献引用格式
Mouse RANKL/TNFSF11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0843)
背景资料
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0843-CAP | 96-well plate precoated with anti- mouse TNFSF11 antibody | 1 |
EK0843-ST | Lyophilized recombinant mouse TNFSF11 standard | 10ng/tube×2 |
EK0843-DA | Biotinylated anti- mouse TNFSF11 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 195 | 430 | 32 | 199 | 419 |
Standard deviation | 1.42 | 10.72 | 17.63 | 1.72 | 12.13 | 21.36 |
CV(%) | 4.2% | 5.5% | 4.1% | 5.4% | 6.1% | 5.1% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.011 | 0.090 | 0.169 | 0.318 | 0.581 | 1.077 | 1.665 | 2.359 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)