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Mouse RANKL/TNFSF11 ELISA Kit

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EK0843

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse RANKL/TNFSF11 ELISA Kit
规格/价格
96T/2480
指标别名
CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE
免疫原
Expression system for standard: NS0; Immunogen sequence: R72-D316
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6pg/ml-1000pg/ml
基因名称
TNFSF11
基因名全称
Tumor necrosis factor ligand superfamily member 11
Uniprot ID
O35235  
文献引用格式
Mouse RANKL/TNFSF11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0843)
背景资料
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
试剂盒内容
Catalog numberDescriptionQuantity
EK0843-CAP96-well plate precoated with anti- mouse TNFSF11 antibody1
EK0843-STLyophilized recombinant mouse TNFSF11 standard10ng/tube×2
EK0843-DABiotinylated anti- mouse TNFSF11 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3419543032199419
Standard deviation1.4210.7217.631.7212.1321.36
CV(%)4.2%5.5%4.1%5.4%6.1%5.1%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0110.0900.1690.3180.5811.0771.6652.359
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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