Anti-CD44 Antibody (Clone#7H7)

筛选器： All WB ICC/IF FCM

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  Figure 1. Western blot analysis of anti- CD44 antibody (M00052-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: rat C6 whole cell lysates.Use mouse anti- CD44 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD44 at approximately 82KD. The expected band size for CD44 is at 82KD.

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  Figure 2. ICC analysis using anti- CD44 antibody (M00052-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).

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  Figure 3. Flow Cytometry analysis of U87 cells using anti-CD44 antibody (M00052-3).
  Overlay histogram showing U87 cells stained with M00052-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-CD44 Antibody (M00052-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of anti- CD44 antibody (M00052-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: rat C6 whole cell lysates.Use mouse anti- CD44 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD44 at approximately 82KD. The expected band size for CD44 is at 82KD.

• 

  Figure 2. ICC analysis using anti- CD44 antibody (M00052-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).

• 

  Figure 3. Flow Cytometry analysis of U87 cells using anti-CD44 antibody (M00052-3).
  Overlay histogram showing U87 cells stained with M00052-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-CD44 Antibody (M00052-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.