Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:50 |
Flow Cytometry (FCM): | 1:50 |
Figure 1. Western blot analysis of anti-CD44 antibody (M00052-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87 MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD44 antigen affinity purified monoclonal antibody (M00052-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.
Figure 1. Western blot analysis of anti-CD44 antibody (M00052-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87 MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD44 antigen affinity purified monoclonal antibody (M00052-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.