Western blot (WB): | 1:400~8000 |
Immunohistochemistry (IHC): | 1:150 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Flow cytometry (FCM): | 1:50 |
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 4. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 5. Anti-CD44 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 .
Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 7. Flow cytometric Analysis of living Colo205 cells, using anti-CD44 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44.
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 4. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 5. Anti-CD44 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 .
Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD44 mouse monoclonal antibody. (M00052-5; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Figure 7. Flow cytometric Analysis of living Colo205 cells, using anti-CD44 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44.