Anti-IDH2 Antibody (Clone#2D4)

筛选器： All WB ICC/IF FCM

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  Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HELA whole cell lysates,
  Lane 2: human SW620 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human HEPG2 whole cell lysates,
  Lane 5: human Jurkat whole cell lysates,
  Lane 6: rat heart tissue lysates,
  Lane 7: rat liver tissue lysates,
  Lane 8: rat PC-12 whole cell lysates,
  Lane 9: mouse heart tissue lysates,
  Lane 10: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.

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  IF analysis of IDH2 using anti-IDH2 antibody (M00510-2).
  IDH2 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-IDH2 Antibody (M00510-2) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of SiHa cells using anti-IDH2 antibody (M00510-2).
  Overlay histogram showing SiHa cells stained with M00510-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH2 Antibody (M00510-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HELA whole cell lysates,
  Lane 2: human SW620 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human HEPG2 whole cell lysates,
  Lane 5: human Jurkat whole cell lysates,
  Lane 6: rat heart tissue lysates,
  Lane 7: rat liver tissue lysates,
  Lane 8: rat PC-12 whole cell lysates,
  Lane 9: mouse heart tissue lysates,
  Lane 10: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.

• 

  IF analysis of IDH2 using anti-IDH2 antibody (M00510-2).
  IDH2 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-IDH2 Antibody (M00510-2) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of SiHa cells using anti-IDH2 antibody (M00510-2).
  Overlay histogram showing SiHa cells stained with M00510-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH2 Antibody (M00510-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.