Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section IHC | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-3).
IDH2 was detected in a paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
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