Anti-IDH2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB9602

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IDH2 using anti-IDH2 antibody (PB9602). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IDH2 antigen affinity purified polyclonal antibody (PB9602) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 45, 51 kDa.
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IHC analysis of IDH2 using anti-IDH2 antibody (PB9602).
IDH2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-IDH2 Antibody (PB9602) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of IDH2 using anti-IDH2 antibody (PB9602).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-IDH2 Antibody (PB9602) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of IDH2 using anti-IDH2 antibody (PB9602).
IDH2 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-IDH2 Antibody (PB9602) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of IDH2 using anti-IDH2 antibody (PB9602).
IDH2 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-IDH2 Antibody (PB9602) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of IDH2 using anti-IDH2 antibody (PB9602).
IDH2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-IDH2 Antibody (PB9602) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IDH2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ICD M; IDH; IDH2; IDHM; IDP; IDPM; mNADP IDH; NADP(+) specific ICDH; Oxalosuccinate decarboxylase

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

IDH2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2, identical to the related mouse and rat sequences.

计算分子量

45, 51 kDa

实际分子量

45 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

Uniprot ID

P48735

文献引用格式

IDH2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9602)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

isocitrate dehydrogenase 2 (NADP+), mitochondrial

蛋白名全称

Isocitrate dehydrogenase [NADP], mitochondrial

实验方案>

实验步骤

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