Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of SiHa cells using anti-IDH2 antibody (M00510-4).
Overlay histogram showing SiHa cells stained with M00510-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH2 Antibody (M00510-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of SiHa cells using anti-IDH2 antibody (M00510-4).
Overlay histogram showing SiHa cells stained with M00510-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH2 Antibody (M00510-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of IDH2 using anti-IDH2 antibody (M00510-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat PC-12 whole cell lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDH2 antigen affinity purified monoclonal antibody (M00510-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of IDH2 using anti-IDH2 antibody (M00510-4).
IDH2 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-IDH2 Antibody (M00510-4) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of SiHa cells using anti-IDH2 antibody (M00510-4).
Overlay histogram showing SiHa cells stained with M00510-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH2 Antibody (M00510-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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