Anti-IL10 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

RP1014

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IL10 using anti-IL10 antibody (RP1014).
Lane 1: recombinant human IL10 protein 10 ng,
Lane 2: recombinant human IL10 protein 5 ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (RP1014) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL10 at approximately 17 kDa.
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IHC analysis of IL10 using anti-IL10 antibody (RP1014).
IL10 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-IL10 Antibody (RP1014) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of IL10 using anti-IL10 antibody (RP1014).
IL10 was detected in a paraffin-embedded section of human appendix tissue. The tissue section was incubated with rabbit anti-IL10 Antibody (RP1014) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-IL10 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ELISA

基因名称

IL10

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human IL-10 recombinant protein(Position: S19-N178).

计算分子量

21 kDa

实际分子量

17 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-10(IL-10 or IL10), also known as human cytokine synthesis inhibitory factor(CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al.(1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al.(1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Uniprot ID

P22301

RRID

AB_3082345

文献引用格式

IL10 Antibody (Boster Biological Technology, Wuhan, China. Catalog#RP1014)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 10

蛋白名全称

Interleukin-10(IL-10)

实验方案>

实验步骤

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