Anti-ACC1/ACACA(Phospho-S80) Antibody (Clone#28A12)

筛选器： All WB

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  Western blot analysis of Phospho-ACC(S80) expression in (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate.

Anti-AKT1 (Phospho-S124) Antibody (Clone#HIA-1)

筛选器： All WB IP

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  Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AKT1 (Phospho-S129) Antibody (Clone#HGD-1)

筛选器： All WB

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  Western blot analysis of Phospho-AKT1 (S129) expression in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AKT1 (Phospho-T450) Antibody (Clone#HEA-1)

筛选器： All WB IHC ICC/IF IP

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  Figure 1. Western blot analysis of anti-AKT1 antibody (BM4721). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (BM4721) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.

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  Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 6. IF analysis of AKT1 using anti-AKT1 antibody (BM4721) and anti-Beta Tubulin antibody (M01857-3).
  AKT1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

Anti-AKT1/3 (Phospho-Y437/434) Antibody

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  Figure 1. Western blotting validation for Anti-Phospho-Akt1/3 (Y437/434) Antibody A00024Y437

  Western Blot (WB) analysis of 293T and HeLa cells using Anti-Phospho-Akt1/3 (Y437/434) polyclonal antibody.
  Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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  Figure 2. Western blotting validation for Anti-Phospho-Akt1/3 (Y437/434) Antibody A00024Y437

  Western Blot (WB) analysis of 293T cells using Anti-Phospho-Akt1/3 (Y437/434) polyclonal antibody.
  Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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  Figure 3. Western blotting validation for Anti-Phospho-Akt1/3 (Y437/434) Antibody A00024Y437

  Western blot (WB) analysis of 293T

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  Figure 4. Immunohistochemistry validation of AKT1 using Anti-Phospho-Akt1/3 (Y437/434) Antibody (A00024Y437).

  Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Phospho-Akt1/3 (Y437/434) polyclonal antibody. Antibody was diluted at 1:100 (4°C

Anti-AMPK Alpha 1/PRKAA1 (Phospho-S496) Antibody (Clone#HDH-16)

筛选器： All WB ICC/IF IP

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  Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.

Anti-AMPK Alpha 2/PRKAA2 (Phospho-S345) Antibody (Clone#HFA-16)

筛选器： All WB

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  Western blot analysis of Phospho-AMPK alpha 2 (S345) expression in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AMPK Alpha 2/PRKAA2 (Phospho-S491) Antibody (Clone#HFI-16)

筛选器： All WB

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  Western blot analysis of AMPK beta 1 expression in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-ATF2 (Phospho-T71) Antibody (Clone#FOO-1)

筛选器： All WB ICC/IF IP

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  Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.

Anti-ATM (Phospho-S1981) Antibody (Clone#AEE-1)

筛选器： All WB IHC ICC/IF IP

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  Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin.

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  Immunohistochemical analysis of paraffin-embedded human liver, using Phospho-ATM (S1981) Antibody.

Anti-B23/NPM1 (Phospho-T199) Antibody (Clone#28N18)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of Phospho-Nucleophosmin (T199) expression in (1) HeLa cell lysate; (2) HeLa cell treated with CA lysate.

Anti-BAD (Phospho-S112) Antibody (Clone#AEBH-2)

筛选器： All WB IP

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  Western blot analysis of Phospho-Bad (S112) expression in (1) HeLa cell lysate; (2) HeLa cell treated with Calcyculin A lysate.

Anti-Beta Catenin/CTNNB1 (PhosphoS33/S37) Antibody (Clone#ICG-3)

筛选器： All WB

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  Western blot analysis of Phospho-beta Catenin (S33/S37) expression in (1) 293T cell lysate; (2) 293T cell lysate treated with calyculin A.

Anti-BRAF (Phospho-T401) Antibody (Clone#IBE-2)

筛选器： All WB ICC/IF IP

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  Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA.

Anti-BTK (Phospho-Y223) Antibody (Clone#ECD-2)

筛选器： All WB IP

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  Western blot analysis of Phospho-BTK (Y223) expression in Raji cell lysate treated with pervanadate.

Anti-BTK (Phospho-Y551) Antibody (Clone#FII-2)

筛选器： All WB ICC/IF

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  Western blot analysis of Phospho-BTK (Y551) expression in Ramos cell lysate treated with Pervanadate.

Anti-c-Cbl/CBL (Phospho-S669) Antibody (Clone#28C46)

筛选器： All WB

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  Western blot analysis of Phospho-CBL (S669) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate lysate.

Anti-c-Cbl/CBL (Phospho-Y774) Antibody (Clone#28C29)

筛选器： All WB IHC FCM

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  Western blot analysis of Phospho-CBL (Y774) expression in Jurkat cell treated with Pervanadate lysate.

Anti-c-Jun (Phospho-S73)+JunD (Phospho-S100) Antibody (Clone#30J61)

筛选器： All WB IHC IP

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  Western blot analysis of Phospho-c-Jun (S73)+JunD (S100) expression in RAW 264.7 cell lysate.

Anti-c-Jun/JUN (Phospho-S63) Antibody (Clone#IGA-10)

筛选器： All WB IHC ICC/IF

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  Western blot analysis of c-Jun phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (S63) Antibody.

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  Immunofluorescent analysis using the Antibody.

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  Immunofluorescent analysis of HeLa cells treated with anisomycin, using Phospho-c-Jun (S63) Antibody.