Anti-ACC1/ACACA(Phospho-S80) Antibody (Clone#28A12)

筛选器： All WB

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  Western blot analysis of Phospho-ACC(S80) expression in (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate.

Anti-AKT1 (Phospho-S124) Antibody (Clone#HIA-1)

筛选器： All WB IP

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  Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-AKT1 (Phospho-S129) Antibody (Clone#HGD-1)

筛选器： All WB

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  Western blot analysis of Phospho-AKT1 (S129) expression in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AKT1 (Phospho-T450) Antibody (Clone#HEA-1)

筛选器： All WB IHC ICC/IF IP

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  Western blot analysis of anti-AKT1 antibody (BM4721). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (BM4721) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.

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  IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  ICC/IF analysis of AKT1 using anti-AKT1 antibody (BM4721) and anti-Beta Tubulin antibody (M01857-3).
  AKT1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

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Anti-AMPK Alpha 1/PRKAA1 (Phospho-S496) Antibody (Clone#HDH-16)

筛选器： All WB ICC/IF IP

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  Western blot analysis of anti-AMPK Alpha 1/PRKAA1 (Phospho-S496) antibody (BM4718). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: monkey COS-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Alpha 1/PRKAA1 (Phospho-S496) antigen affinity purified monoclonal antibody (BM4718) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Alpha 1/PRKAA1 (Phospho-S496) at approximately 64 kDa. The expected band size for AMPK Alpha 1/PRKAA1 (Phospho-S496) is at 64 kDa.

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Anti-AMPK Alpha 1/PRKAA1 (Phospho-T183)/AMPK Alpha 2/PRKAA2 (Phospho-T172) Antibody (Clone#31P05)

筛选器： All WB IHC

Anti-AMPK Alpha 2/PRKAA2 (Phospho-S345) Antibody (Clone#HFA-16)

筛选器： All WB

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  Western blot analysis of Phospho-AMPK alpha 2 (S345) expression in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.