Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebra fish tissue lysates,
Lane 2: chicken heart tissue lysates,
Lane 3: chicken liver tissue lysates,
Lane 4: chicken brain tissue lysates,
Lane 5: monkey heart tissue lysates,
Lane 6: monkey lung tissue lysates,
Lane 7: monkey kidney tissue lysates,
Lane 8: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 1. Western blot analysis of anti- β-Actin antibody (BM0627).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat brain tissue lysates,Lane 6: Rat PC-12 whole cell lysates,Lane 7: Mouse brain tissue lysates,Lane 8: Mouse ANA-1 whole cell lysates,Lane 9: Rabbit intestines tissue lysates,Lane 10: Rabbit intestines tissue lysates,Lane 11: Pig intestines tissue lysates.Use rabbit anti- β-Actin 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.

Figure 2.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Mouse Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 3.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Rat Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 4.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Intestinal Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 5.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Lung Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 6.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BM3873). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human GES-1 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse PC-12 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified monoclonal antibody (BM3873) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 1. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human Hacat whole cell lysates,
Lane 6: human HUVEC whole cell lysates,
Lane 7: human SK-OV-3 whole cell lysates,
Lane 8: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:10000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Figure 2. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat NRK whole cell lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:10000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Figure 1. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Figure 2. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat RH-35 whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.

Figure 1. Western blot analysis of anti- alpha Tubulin antibody (BM1452). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SH-SY5Y whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse thymus tissue lysates.
Use mouse anti- alpha Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for alpha Tubulin at approximately 55KD. The expected band size for alpha Tubulin is at 50KD.

Figure 2. IHC analysis using anti- alpha Tubulin antibody (BM1452). detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of anti-Alpha Tubulin/TUBA1B antibody (BM3885). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Tubulin/TUBA1B antigen affinity purified monoclonal antibody (BM3885) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Tubulin/TUBA1B at approximately 55 kDa. The expected band size for Alpha Tubulin/TUBA1B is at 50 kDa.

Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.

Figure 1. Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue (37℃) lysates,
Lane 2: rat brain tissue (100℃) lysates,
Lane 3: mouse brain tissue (37℃) lysates,
Lane 4: mouse brain tissue (100℃) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP1A1 antigen affinity purified polyclonal antibody (PB0504) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATP1A1 at approximately 100 kDa. The expected band size for ATP1A1 is at 113 kDa.

Figure 2. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 5. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 6. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 7. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 8. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 9. IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody.

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using Sodium Potassium ATPase Antibody.

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human cervical cancer, using the Antibody.

Immunofluorescent analysis using the Antibody.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (A01263-HRP). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (A01263-HRP). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (A01263-HRP). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (A01263-HRP). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 1. Western blot analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: chicken heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Tubulin/TUBB antigen affinity purified polyclonal antibody (A01857-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Tubulin/TUBB at approximately 50 kDa. The expected band size for Beta Tubulin/TUBB is at 50 kDa.

Figure 2. IHC analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1) .
Beta Tubulin/TUBB was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1) .
Beta Tubulin/TUBB was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IF analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1).
Beta Tubulin/TUBB was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Figure 5. Flow Cytometry analysis of SiHa cells using anti-Beta Tubulin/TUBB antibody (A01857-1).
Overlay histogram showing SiHa cells stained with A01857-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 1.IHC analysis using anti-BrdU antibody (BM0201). detected in paraffin-embedded section of HELA cells. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of anti- COX4I1 antibody (M05442-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human T47D whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates.
Use mouse anti- COX4I1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for COX4I1 at approximately 17KD. The expected band size for COX4I1 is at 20KD.

Figure 2. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human colon cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

Figure 3. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

Figure 4. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

Figure 5. Flow Cytometry analysis of U937 cells using anti-COX4I1 antibody (M05442-1).
Overlay histogram showing U937 cells stained with M05442-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COX4I1 Antibody (M05442-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Figure 1. Western blot analysis of COX4I1 using anti-COX4I1 antibody (A05442-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX4I1 antigen affinity purified polyclonal antibody (A05442-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.

Figure 2. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat liver tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat lung tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 5. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 6. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 7. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human placenta tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 8. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 9. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse kidney tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 10. ICC analysis of anti-COX4I1 antibody (A05442-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).

Figure 11. Flow Cytometry analysis of U937 cells using anti-COX4I1 antibody (A05442-1).
Overlay histogram showing U937 cells stained with A05442-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX4I1 Antibody (A05442-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Figure 1. Western blot analysis of anti-COX4I1 antibody (BM4046). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX4I1 antigen affinity purified monoclonal antibody (BM4046) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.

Figure 2. IHC analysis of COX4I1 using anti-COX4I1 antibody (BM4046) .
COX4I1 was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with rabbit anti-COX4I1 Antibody (BM4046) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of COX4I1 using anti-COX4I1 antibody (BM4046) .
COX4I1 was detected in a paraffin-embedded section of human live cancer tissue. The tissue section was incubated with rabbit anti-COX4I1 Antibody (BM4046) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IHC analysis of COX4I1 using anti-COX4I1 antibody (BM4046) .
COX4I1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-COX4I1 Antibody (BM4046) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 1. Western blot analysis of anti-GAPDH antibody (BA2913). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human CCRF-CEM whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat smooth muscle tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (BA2913) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Figure 2. Western blot analysis of anti-GAPDH antibody (BA2913). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: chicken heart tissue lysates,
Lane 2: chicken liver tissue lysates,
Lane 3: chicken brain tissue lysates,
Lane 4: monkey heart tissue lysates,
Lane 5: monkey lung tissue lysates,
Lane 6: monkey kidney tissue lysates,
Lane 7: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (BA2913) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Figure 3. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 5. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 6. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human testicular cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 7. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 8. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 9. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 1. Western blot analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).
The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human 22RV1 whole cell lysates,Lane 4: human CACO-2 whole cell lysates,Lane 5: human CCRF-CEM whole cell lysates,Lane 6: human HEPG2 whole cell lysates,Lane 7: human THP-1 whole cell lysates,Lane 8: rat PC-12 whole cell lysates,Lane 9: mouse HEPA1/6 whole cell lysates,Lane 10: mouse NIH/3T3 whole cell lysates.
anti-Histone H3 antigen affinity purified polyclonal antibody (Catalog # A12477-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Histone H3 at approximately 17KD. The expected band size for Histone H3 is at 15KD.

Figure 2. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 5. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 6. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 7. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 8. IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2).Histone H3 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 10. IF analysis of Histone H3 and GFAP using anti-Histone H3 antibody (A12477-2) and anti-GFAP antibody (M00213-8).Histone H3 and GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Histone H3 antibody (A12477-2) and mouse anti-GFAP antibody (M00213-8) overnight at 4°C. DyLight?488 Conjugated Goat Anti-Rabbit IgG (BA1127), Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 9. ICC analysis using anti- Histone H3 antibody (A12477-2). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody.

Immunofluorescent analysis of Hela cells, using Histone H3 Antibody.

Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.

All lanes use the Antibody for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded Rat thymus, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Mouse spleen, using the Antibody.

Immunohistochemical analysis of paraffin-embedded mouse brain, using Histone H3 Antibody.

Immunohistochemical analysis of paraffin-embedded Human tonsil, using the Antibody.

Figure 1. Western blot analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (PB9337). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP60/HSPD1 antigen affinity purified polyclonal antibody (PB9337) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSP60/HSPD1 at approximately 61 kDa. The expected band size for HSP60/HSPD1 is at 61 kDa.

Figure 2. IHC analysis of Hsp60 using anti-Hsp60 antibody (PB9337).Hsp60 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Hsp60 using anti-Hsp60 antibody (PB9337).Hsp60 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of Hsp60 using anti-Hsp60 antibody (PB9337).Hsp60 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 5. ICC analysis of Hsp60 using anti-Hsp60 antibody (PB9337).
Hsp60 was detected in immunocytochemical section of SMMC-7721 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 6. IF analysis of Hsp60 using anti-Hsp60 antibody (PB9337).
Hsp60 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 1. Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane 1: human Caco-2 whole cell lysates

Lane 2: human A549 whole cell lysates

Lane 3: human THP-1 whole cell lysates

Lane 4: human SW620 whole cell lysates

Lane 5: human U-937 whole cell lysates

Lane 6: human HepG2 whole cell lysates

Lane 7: rat RH35 whole cell lysates

Lane 8: mouse RAW246.7 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPD1 antigen affinity purified monoclonal antibody (Catalog # M01280-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.

Figure 2. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
HSPD1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
HSPD1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 4. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
HSPD1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 5. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
HSPD1 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 6. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
HSPD1 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 7. Flow Cytometry analysis of A431 cells using anti-HSP60/HSPD1 antibody (M01280-3).
Overlay histogram showing A431 cells stained with M01280-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSP60/HSPD1 Antibody (M01280-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Figure 8. Flow Cytometry analysis of HepG2 cells using anti-HSP60/HSPD1 antibody (M01280-3).
Overlay histogram showing HepG2 cells stained with M01280-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSP60/HSPD1 Antibody (M01280-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Figure 9. ICC analysis using anti- HSPD1 antibody (M01280-3). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).