Human TNF Alpha/TNFA ELISA Kit

96T/1880

Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor

E.coli, V77-L233

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

15.6 pg/ml - 1000 pg/ml

TNF

tumor necrosis factor

P01375

Human TNF Alpha/TNFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0525)

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

20-25分钟

Catalog number	Description	Quantity
EK0525-CAP	Anti-Human TNF Pre-coated 96-well strip microplate	1
EK0525-ST	Human TNF Standard	10ng/tube×2
EK0525-DA	Human TNF Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	16	82	201	14	75	186
Standard deviation	0.76	5.49	12.66	0.75	5.55	11.9
CV(%)	4.8	6.7	6.3	5.4	7.4	6.4

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.065	0.118	0.167	0.272	0.510	0.839	1.582	2.412

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h