Mouse TNF Alpha/TNFA Quick ELISA Kit

96T/3580

Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor

Expression system for standard: E.coli; Immunogen sequence: L80-L235

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum plasma)

TNF

tumor necrosis factor

P16599

Mouse TNF Alpha/TNFA Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0527)

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

15分钟

Catalog number	Description	Quantity
FEK0527-CAP	Anti-mouse TNFα Pre-coated 96-well Strip Microplate	1
FEK0527-ST	mouse TNFα standard	10ng/tube×2
FEK0527-H	HRP Linked Anti-mouse TNFα Antibody	6ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	14	96	280	13	98	306
Standard deviation	0.57	7.39	20.44	0.78	7.54	24.78
CV(%)	4.1%	7.7%	7.3%	6%	7.7%	8.1%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.160	0.232	0.311	0.406	0.654	1.065	1.673	2.430

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)