Rat TNF Alpha/TNFA ELISA Kit

96T/2480

Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor

Expression system for standard: E.coli; Immunogen sequence: L80-L235

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

15.6pg/ml-1000pg/ml

TNF

tumor necrosis factor

P16599

Rat TNF Alpha/TNFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0526)

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

20-25分钟

Catalog number	Description	Quantity
EK0526-CAP	96-well plate precoated with anti- rat TNF alpha antibody	1
EK0526-ST	Lyophilized recombinant rat TNF alpha standard	10ng/tubex2
EK0526-DA	Biotinylated anti- rat TNF alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	14	96	280	13	98	306
Standard deviation	0.57	7.39	20.44	0.78	7.54	24.78
CV(%)	4.1%	7.7%	7.3%	6%	7.7%	8.1%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.010	0.061	0.117	0.225	0.443	0.833	1.645	2.481

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)