Anti-AMPK Beta 2/PRKAB2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ICC/IF

PB0723

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IF analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723).
AMPK Beta 2/PRKAB2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-AMPK Beta 2/PRKAB2 Antibody (PB0723) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Western blot analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Rat Skeletal Muscle tissue lysates,
Lane 3: PANC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Beta 2/PRKAB2 antigen affinity purified polyclonal antibody (PB0723) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Beta 2/PRKAB2 at approximately 34 kDa. The expected band size for AMPK Beta 2/PRKAB2 is at 30 kDa.
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IHC analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723).
AMPK Beta 2/PRKAB2 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AMPK Beta 2/PRKAB2 Antibody (PB0723) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723).
AMPK Beta 2/PRKAB2 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AMPK Beta 2/PRKAB2 Antibody (PB0723) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723).
AMPK Beta 2/PRKAB2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AMPK Beta 2/PRKAB2 Antibody (PB0723) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of A431 cells using anti-AMPK Beta 2/PRKAB2 antibody (PB0723).
Overlay histogram showing A431 cells stained with PB0723 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK Beta 2/PRKAB2 Antibody (PB0723) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(6)
IF analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723).
AMPK Beta 2/PRKAB2 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-AMPK Beta 2/PRKAB2 Antibody (PB0723) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
Western blot analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (PB0723). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Rat Skeletal Muscle tissue lysates,
Lane 3: PANC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Beta 2/PRKAB2 antigen affinity purified polyclonal antibody (PB0723) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Beta 2/PRKAB2 at approximately 34 kDa. The expected band size for AMPK Beta 2/PRKAB2 is at 30 kDa.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-AMPK Beta 2/PRKAB2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AMPK beta 2; AMPK subunit beta 2; PRKAB2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ICC/IF

基因名称

PRKAB2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2 different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

计算分子量

30 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

Uniprot ID

O43741

文献引用格式

AMPK Beta 2/PRKAB2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0723)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

protein kinase, AMP-activated, beta 2 non-catalytic subunit

蛋白名全称

5'-AMP-activated protein kinase subunit beta-2

实验方案>

实验步骤

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