Figure 1. Western blot analysis of anti-14-3-3 alpha/beta antibody (BM4752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 alpha/beta antigen affinity purified monoclonal antibody (BM4752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 alpha/beta at approximately 28 kDa. The expected band size for 14-3-3 alpha/beta is at 28 kDa.

Western blot analysis of 14-3-3 expression in Hela lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 Antibody.

Figure 1. Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: SH-SY5Y whole cell lysates,
Lane 3: SW620 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-14-3-3 Epsilon/YWHAE antigen affinity purified monoclonal antibody (M01687-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Epsilon/YWHAE at approximately 29 kDa. The expected band size for 14-3-3 Epsilon/YWHAE is at 29 kDa.

Figure 2. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 5. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human endometrial cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 6. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 7. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 8. IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

Figure 10. Flow Cytometry analysis of ANA-1 cells using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
Overlay histogram showing ANA-1 cells stained with M01687-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Figure 11. Flow Cytometry analysis of NRK cells using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
Overlay histogram showing NRK cells stained with M01687-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Figure 9. IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:100. Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate.

Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.

Western blot analysis of 14-3-3 sigma expression in A431 cell lysate.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

Western blot analysis of 14-3-3 Theta expression in HeLa cell lysate.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Figure 1. Western blot analysis of anti-5 Lipoxygenase/ALOX5 antibody (BM5522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified monoclonal antibody (BM5522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.

Western blot analysis of ASPP2 expression in MCF7 cell lysate.

Figure 1. Western blot analysis of anti-α-SMA antibody (BM0002). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-α-SMA antigen affinity purified polyclonal antibody (BM0002) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for α-SMA at approximately 42 kDa. The expected band size for α-SMA is at 42 kDa.

Figure 2. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 5. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of human endometrial carcinoma tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 6. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 7. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 8. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of rat heart muscle tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 9. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of rat lung tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with AEC (Catalog # AR1020) as the chromogen.

Figure 10. IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.

Figure 11. IF analysis of α-SMA using anti-α-SMA antibody (BM0002).
α-SMA was detected in a paraffin-embedded section of rat lung tissue. Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Figure 12. IF analysis of α-SMA using anti-α-SMA antibody (BM0002) and anti Anti-PECAM-1/CD31 antibody (PB9094).
α-SMA was detected in a paraffin-embedded section of human placenta tissue. Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog#BA1126) and Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Figure 1. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM4172). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM4172) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

Figure 1. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U251 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

Figure 2. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .

Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POTEG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POTEG (1:2000).

Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBFOX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBFOX1.

Western blot analysis of AAMP expression in (1) A375 lysate; (2) MCF7 cell lysate.

Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AANAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT (1:2000).

Western blot analysis of ABAT expression in HepG2 cell lysate.

Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.

Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M07133-3)

Figure 3. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M07133-3)