Anti-CD44 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A00052-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD44 using anti-CD44 antibody (A00052-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (A00052-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD44 at approximately 85 kDa. The expected band size for CD44 is at 82 kDa.
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IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of mouse lymphaden tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of CD44 using anti-CD44 antibody (A00052-2).
CD44 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of MCF-7 cells using anti-CD44 antibody (A00052-2).
Overlay histogram showing MCF-7 cells stained with A00052-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (A00052-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD44 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD44; LHR; MDU2; MDU3; MIC4; CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD antigen CD44

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

CD44

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD44 recombinant protein (Position: Q21-E518). Human CD44 shares 66.5% amino acid (aa) sequence identity with mouse CD44.

计算分子量

82 kDa

实际分子量

82 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Uniprot ID

P16070

Gene ID

960

文献引用格式

CD44 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00052-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD44 molecule (IN blood group)

蛋白名全称

CD44 antigen

实验方案>

实验步骤

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