Anti-CARD4/NOD1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB FCM IHC ELISA

A00495-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (A00495-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody (A00495-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CARD4/NOD1 at approximately 107 kDa. The expected band size for CARD4/NOD1 is at 108 kDa.
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Flow Cytometry analysis of Caco-2 cells using anti-CARD4/NOD1 antibody (A00495-2).
Overlay histogram showing Caco-2 cells stained with A00495-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CARD4/NOD1 Antibody (A00495-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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IHC analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (A00495-2).
CARD4/NOD1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CARD4/NOD1 Antibody (A00495-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-CARD4/NOD1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

nucleotide binding oligomerization domain containing 1; CARD4; CLR7.1; NLRC1; NOD1

产品类型

Polyclonal

检验物种

human

应用范围

WB, FCM, IHC, ELISA

基因名称

NOD1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CARD4/NOD1 recombinant protein (Position: N26-F953).

计算分子量

108 kDa

实际分子量

107 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is mapped to 7p14.3. It recognizes bacterial molecules and stimulates an immune reaction. NOD1 protein contains a caspase recruitment domain (CARD). This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. What wore, it has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.

Uniprot ID

Q9Y239

文献引用格式

CARD4/NOD1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00495-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nucleotide binding oligomerization domain containing 1

蛋白名全称

Nucleotide-binding oligomerization domain-containing protein 1

实验方案>

实验步骤

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