Anti-NOTCH2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A00518-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOTCH2 antigen affinity purified polyclonal antibody (A00518-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOTCH2 at approximately 110, 300 kDa. The expected band size for NOTCH2 is at 110 kDa.
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IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2).
NOTCH2 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was incubated with rabbit anti-NOTCH2 Antibody (A00518-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2).
NOTCH2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-NOTCH2 Antibody (A00518-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2).
NOTCH2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NOTCH2 Antibody (A00518-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2).
NOTCH2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NOTCH2 Antibody (A00518-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2).
NOTCH2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-NOTCH2 Antibody (A00518-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of SH-SY5Y cells using anti-NOTCH2 antibody (A00518-2).
Overlay histogram showing SH-SY5Y cells stained with A00518-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOTCH2 Antibody (A00518-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NOTCH2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AGS2; hN2; Notch 2; NOTCH2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

NOTCH2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human NOTCH2 recombinant protein (Position: H2194-A2471).

计算分子量

110 kDa

实际分子量

110, 300 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Neurogenic locus notch homolog protein 2 also known as notch 2 is a protein that in humans is encoded by the NOTCH2 gene. It is mapped to 1p12. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

Q04721

文献引用格式

NOTCH2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00518-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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