Western blot (WB): | 1:500-2000 |
Western blot analysis of DKK1 using anti-DKK1 antibody (A00632).
Lane 1: recombinant human DKK1 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (A00632) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DKK1 at approximately 38 kDa.
Western blot analysis of DKK1 using anti-DKK1 antibody (A00632).
Lane 1: recombinant human DKK1 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (A00632) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DKK1 at approximately 38 kDa.