Anti-TCF4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00674-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TCF4 using anti-TCF4 antibody (A00674-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SH-SY5Y whole cell lysates,
Lane 2: Daudi whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TCF4 antigen affinity purified polyclonal antibody (A00674-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TCF4 at approximately 71 kDa. The expected band size for TCF4 is at 71 kDa.
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产品名称

Anti-TCF4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

bHLHb19; E2 2; ITF 2; ITF2; PTHS; SEF 2; SEF2; SEF2 1; SEF2 1A; SEF2 1B; SL3 3 enhancer factor 2; TCF 4; TCF4; transcription factor 4

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

TCF4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human TCF4 recombinant protein (Position: Q147-V176).

计算分子量

71 kDa

实际分子量

71 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Transcription factor 4 (TCF-4) also known as immunoglobulin transcription factor 2 (ITF-2) is a protein that in humans is encoded by the TCF4 gene located on chromosome 18q21.2. This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Uniprot ID

P15884

文献引用格式

TCF4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00674-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transcription factor 4

蛋白名全称

Transcription factor 4

实验方案>

实验步骤

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