Anti-Cytoglobin/CYGB Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A01197

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat small intestine tissue lysates,
Lane 2: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytoglobin/CYGB antigen affinity purified polyclonal antibody (A01197) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytoglobin/CYGB at approximately 21 kDa. The expected band size for Cytoglobin/CYGB is at 21 kDa.
all(6)
IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
Cytoglobin/CYGB was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
Cytoglobin/CYGB was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
Cytoglobin/CYGB was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
Cytoglobin/CYGB was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
Cytoglobin/CYGB was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)

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产品简介>

产品名称

Anti-Cytoglobin/CYGB Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CYGB; cytoglobin; HGB; Histoglobin; STAP

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CYGB

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Cytoglobin recombinant protein (Position: M1-P190). Human Cytoglobin shares 95.3% and 93.7% amino acid (aa) sequence identity with mouse and rat Cytoglobin, respectively.

计算分子量

21 kDa

实际分子量

21 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cytoglobin(CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir.

Uniprot ID

Q8WWM9

文献引用格式

Cytoglobin/CYGB Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01197)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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