Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (A02296-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (A02296-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
IHC analysis of EEA1 using anti-EEA1 antibody (A02296-3) .
EEA1 was detected in a paraffin-embedded section of human breast cancer issue. The tissue section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of EEA1 using anti-EEA1 antibody (A02296-3) .
EEA1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of EEA1 using anti-EEA1 antibody (A02296-3).
EEA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (A02296-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (A02296-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
IHC analysis of EEA1 using anti-EEA1 antibody (A02296-3) .
EEA1 was detected in a paraffin-embedded section of human breast cancer issue. The tissue section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of EEA1 using anti-EEA1 antibody (A02296-3) .
EEA1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of EEA1 using anti-EEA1 antibody (A02296-3).
EEA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (A02296-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (A02296-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
IHC analysis of EEA1 using anti-EEA1 antibody (A02296-3) .
EEA1 was detected in a paraffin-embedded section of human breast cancer issue. The tissue section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of EEA1 using anti-EEA1 antibody (A02296-3) .
EEA1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of EEA1 using anti-EEA1 antibody (A02296-3).
EEA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-EEA1 Antibody (A02296-3) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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