Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of ACOX1 using anti-ACOX1 antibody (A03054-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACOX1 antigen affinity purified polyclonal antibody (A03054-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACOX1 at approximately 22 kDa. The expected band size for ACOX1 is at 74 kDa.
Western blot analysis of ACOX1 using anti-ACOX1 antibody (A03054-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACOX1 antigen affinity purified polyclonal antibody (A03054-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACOX1 at approximately 22 kDa. The expected band size for ACOX1 is at 74 kDa.